The Next-Generation Technology Expediting Mouse Model Creation
TurboMice ™ Technology
Tsim los ntawm MingCeler los ntawm kev ua kom zoo dua ntawm tetraploid ntxiv.
Los ntawm kev sib xyawPrecise Gene Editing technologythiab optimized nasEmbryonic Stem Cell Preparation technology, tam sim no peb tuaj yeem hloov kho yuav luag txhua lub hom phiaj gene locus.
TurboMice ™ Technology
Peb cov cuab yeej ua tau zoo Tetraploid Complementation tau tso cai rau peb ua kom muaj kev yug me nyuam ntawm nas los ntawm 1% -5% mus rau 30% -60% nrog cov gene-edited qia hlwb, ze li qhov ua tau zoo ntawm kev hloov embryo.
MingCeler yog thawj lub tuam txhab nyob hauv lub ntiaj teb kom ua tiav kev hloov pauv ntawm Tetraploid Complementation thev naus laus zis los ntawm chav kuaj mus rau kev lag luam.
Tsoos Technology
Cov tsiaj ua qauv siv thev naus laus zis, xws li pronuclear microinjection thiab ES lub hom phiaj, suav nrog kev yug me nyuam tsawg kawg 2 mus rau 3 tiam kom tau txais nas ntawm homozygous genotype, uas feem ntau yuav siv li 6-8 lub hlis.
Cov txheej txheem ntev no cuam tshuam qhov kev nce qib thiab kev ua haujlwm ntawm kev tsim tshuaj tshiab thiab kev tshawb fawb txog kab mob.
Ceev Batch
1. mus txog 20 homozygous gene-edited nas nyob rau hauv2-4 lub hlis.
2. ≈ 50 homozygous nas nyob hauv qab no2 hli.
3. 400+ homozygous nas nyob hauv8-12 lub hlis.
Case Report hauv 2020
TurboMice ™ thev naus laus zis tau siv los ua kom sai sai500 homozygoushumanized ACE2 nas nyob rau hauv xwb8 muajrau kev tshawb fawb tshuaj thiab tshuaj tiv thaiv COVID-19.
Flexible Strain Selection
Cov txheej txheem ib txwm muaj cov kev txwv tseem ceeb hauv kev xaiv nas hom, thaum TurboMice ™ thev naus laus zis muaj kev hloov pauv ntau dua nrog ntau hom kab mob thiab cov kab mob sib kis los xaiv los ntawm (xws li Balb /c, ICR, C57BL / 6, thiab lwm yam).
● F0 tiam nas tsim los siv TurboMice™ thev naus laus zis yog cov nas lub hom phiaj nrog ib leeg-cell keeb kwm.
● Yog li ntawd, cov khoom siv caj ces ntawm cov nas F0 yog tib yam, txo qhov kev sim ua yuam kev los ntawm cov khoom siv caj ces sib txawv.
● Ergo, kev sim cov ntaub ntawv rau kev soj ntsuam cov tshuaj ua tau zoo thiab lwm yam kev sim tau zoo sib xws thiab txhim khu kev qha.
Ua kom muaj kev ncaj ncees ntawm caj ces zoo
Hauv Situ Precision Gene Editing
● Nyob rau hauv situ meej gene kho nrog cov gene qhia theem thiab meej cov ntaub so ntswg.
● Humanized ACE2 nas tsim los ntawm cov txheej txheem ib txwm muaj yog tsim los ntawm exogenous kev taw qhia ntawm K18-ACE2 txhawb nqa, uas tsis tuaj yeem ua tiav cov kev kho kom raug vim yog qhov tsis sib xws, ua rau tsis muaj cov ntaub so ntswg tshwj xeeb ntawm ACE2 qhia hauv kev tsim humanized ACE2 nas qauv. .
● MingCeler's humanized ACE2 nas qhia tshwj xeeb hauv cov kab mob sib txawv (graphics C thiab D saum toj no), thiab simulate cov yam ntxwv kho mob zoo tom qab tus kab mob SARS-CoV-2.
Unique EnhancerPlus Platform
●MingCeler's proprietary EnhancerPlus platform tuaj yeem pab peb cov neeg siv khoom txhim kho kev qhia qib ntawm tib neeg cov noob.
● Cov txiaj ntsig ntawm ntau xyoo ntawm cov ntaub ntawv khaws cia hauv epigenetics thiab kev txiav txim siab txoj hmoo ntawm tes, MingCeler's EnhancerPlus platform tuaj yeem kwv yees qhov tseeb ntawm genomic txoj haujlwm ntawm Enhancers, ua rau kev tsim cov noob kho cov tswv yim pov thawj raws li.
● Yog li ntawd, lub hom phiaj cov noob tuaj yeem zoo ib yam li cov qauv qhia thiab qib ntawm cov noob caj noob ces.
Case report: Humanization of X noob.
● Leveraging lub EnhancerPlus platform, lub tswv yim yog optimized thiab qhia theem pom ib tug nce ntawm peb qhov kev txiav txim ntawm qhov loj (saib saum toj no cov duab), mus txog qhov yuav tsum tau rau kev loj hlob tshuaj raws li tau txiav txim los ntawm tus neeg muas zaub nyob rau hauv lub protein theem.
● Tom qab ua tiav lub ntiaj teb thawj qhov loj-tsim ntawm ACE2 humanized nas nyob rau hauv 2020, MingCeler tau upgraded ACE2 humanized nas qauv los ntawm plaub iterations ntawm EnhancerPlus optimization, nrog rau theem ntawm humanized ACE2 nws thiaj li mus txog ze rau endogenous qhia ntawm nas ACE2.
Kev nthuav qhia theem ntawm humanized ACE2 nyob rau hauv ntau yam kabmob ntawm humanized nas qauv
Multi-Locus Gene Editing
● TurboMice ™ thev naus laus zis yog qhov kev xaiv zoo tshaj plaws nyob rau hauv kev ua lag luam rau kev tsim ntau qhov chaw combinatorial gene-edited nas qauv, muab qhov zoo ntawm qhov tseeb siab hauv qhov chaw ntxig, qhov ntev ntu ntu, thiab ntau qhov kev hloov pauv caj ces, tsis muaj teeb meem kev sib cais.
● TurboMice ™ thev naus laus zis tso cai rau kev hloov kho ib txhij txog li 3 lub noob los tsim homozygous multi-locus gene-edited nas qauv ncaj qha los ntawm cov noob edited embryonic qia hlwb, tsis tas yuav siv sij hawm siv sij hawm yug me nyuam / kev tshuaj ntsuam, muab ntau yam. ntawm cov qauv uas muaj nuj nqis siab xav tau rau kev tshawb fawb tshuaj tshiab.
● MingCeler tau ua tiav kev tsim ntau yam ntawm ntau hom kab mob gene-edited nas qauv hauv lub sijhawm luv luv, raws li ACE2 humanized nas.
Ntev Fragment Gene Editing
TurboMice ™ thev naus laus zis tso cai rau cov noob hloov kho ntawm cov kab ntev ntev ntawm ntau dua 20kb, ua kom yooj yim rau kev tsim cov qauv nyuaj xws li humanization, conditional knock-out (CKO), thiab loj fragment knock-in (KI).
Kev tshaj tawm
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin khi ACE2 los thaiv nws cov kev cuam tshuam nrog SARS-CoV-2 spike protein thiab muaj txiaj ntsig zoo hauv kev tiv thaiv kab mob SARS-CoV-2 hauv cov qauv tsiaj.Cell Res.2021 Jan; 31(1):17-24.doi: 10.1038/s41422-020-00450-0 ib.(Ib.: 20.507)
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Rapid generation of ACE2 humanized inbred mouse model for COVID-19 with tetraploid complementation.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.(Ib.: 16,693)
Kev pabcuam Flow
Txhua yam koj yuav tsum tau ua yog muab peb koj cov kev hloov pauv noob rau koj tus qauv nas.Raws li koj qhov kev qhia tshwj xeeb, peb yuav muab tso ua ke ib qho kev npaj ua ntej, thiab tom qab kev sib tham ntxiv thiab kev pom zoo, peb yuav kos npe rau daim ntawv cog lus kev pabcuam.Tom qab pib qhov project peb yuav muab cov kev hloov kho raws sij hawm ntawm kev nce qib.