Muas yug
MingCeler muaj cov chaw ua tiav thiab tuaj yeem ua tiav ntau txoj haujlwm yug nas raws li cov neeg siv khoom xav tau.
IVF expansion
Mouse in vitro fertilization (IVF, in vitro fertilization) yog hais txog cov txheej txheem fertilization ntawm nas phev thiab qe nyob rau hauv ib puag ncig tswj kev tsim khoom hauv vitro, thiab cov qe fertilized tau hloov mus rau cov niam txiv pseudopregnant kom tau cov nas yug tshiab.Kev siv hauv vitro fertilization (IVF) thev naus laus zis tuaj yeem txo qis kev siv cov txiv neej nas thiab tuaj yeem tau txais ntau tus nas nyob rau tib lub lim tiam-hnub nyoog.
Hauv vitro fertilization (IVF):
1, txhim kho tus nqi siv ntawm txiv neej nas.
1-2 lub lis piam ntawm cov txiv neej uas muaj hnub nyoog tsim nyog (12-16 lub lis piam) tuaj yeem ua tiav qhov kev sim hauv vitro fertilization, uas txuag cov txiv neej nas thiab txo qis kev siv cov kev sim.
2, shorten lub sij hawm yug me nyuam.
Kev yug me nyuam, poj niam nas xav tau 6-8 lub lis piam kom muaj kev sib deev ua ntej lawv tuaj yeem siv rau kev sib deev, thaum poj niam nas siv IVF tuaj yeem superovulated hauv 4 lub lis piam xwb.IVF tsuas yog siv sijhawm 1.5-2 lub hlis, piv rau 3 lub hlis rau ib tiam neeg yug los.
3, txo tus kheej qhov sib txawv ntawm tib batch ntawm cov me nyuam nas.
Hnub yug ntawm tib pawg ntawm cov me nyuam nas tau txais los ntawm in vitro fertilization (IVF) sib txawv los ntawm tsis pub ntau tshaj 5 hnub, txo cov sij hawm vim nas lub lis piam ntawm hnub nyoog thiab batch sib txawv heev txhim kho qhov zoo ntawm kev sim.Rau cov txiv neej nas uas nyuaj rau kev yug me nyuam, IVF tuaj yeem txhim kho qhov kev vam meej ntawm kev yug me nyuam.
Sperm cryopreservation / resuscitation
Cov khoom siv noob caj noob ces tsuas yog siv txoj hauv kev kom khov, uas tuaj yeem khov cov phev, embryo, epididymis, zes qe menyuam, thiab lwm yam. Ntawm lawv, cryopreservation ntawm phev thiab embryos ntawm nas tuaj yeem txo cov nyiaj, sijhawm, thiab qhov chaw nyob los ntawm kev yug tsiaj, thiab tsis txhob kev poob ntawm cov noob hauv cov txheej txheem ntawm kev pub mis.
Cov ntaub ntawv
[1] Esfandiari NGubistaA.Mouse embryo assay rau tib neeg nyob rau hauv vitro fertilization zoo tswj: ib tug tshiab saib.J AssistReprodGenet.2020May;37(5):1123-1127.doi: 10.1007/s10815-020-01768-9.Epub2020 Apr 12. PMID:32281036;PIB: PMC7244663.
[2] Mochida K, Hasegawa AShikataD, Itami N, Hada M, WatanabeN, TomishimaT, Ogura A. Ib qho yooj yim thiab ceev (EQ) phev freezing txoj kev ceev ceev ceev ceev ntawm nas hom.Sci Rep. 2021 Lub Xya Hli 8; 11(1): 14149.doi: 10.1038/s41598-021-93604-y.PIB: 34239008;PIB: PMC8266870.