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Gene-edited cell kab

Gene-edited cell kab xa mus rau cov hlwb uas tau hloov kho caj ces los ntawm cov txheej txheem hu ua gene editing.Kev kho noob caj noob ces suav nrog kev hloov pauv DNA ntawm cov kab mob, feem ntau nrog lub hom phiaj ntawm kev kawm cov noob caj noob ces lossis nkag siab qee yam txheej txheem lom neeg.

Siv cov thev naus laus zis kho tshiab, nws muaj peev xwm ua tiav cov kab ua haujlwm ntawm tes, cov kab tawm ntawm tes, taw tes hloov pauv, lossis khob ntawm cov kab ntawm cov noob hauv cov hlwb tshwj xeeb, uas tuaj yeem siv los kawm cov noob muaj nuj nqi, teeb liab, kab mob. , thiab kev txhim kho tshuaj, nrog rau kev sau cov hlwb tshwj xeeb.

Muaj ob peb lub laj thawj vim li cas gene-edited cell kab muaj txiaj ntsig rau kev tshawb fawb tshawb fawb.Ua ntej, lawv muab cov cuab yeej los kawm txog kev ua haujlwm ntawm cov noob tshwj xeeb.Los ntawm kev hloov cov noob nyob rau hauv ib puag ncig tswj, cov kws tshawb fawb tuaj yeem soj ntsuam cov txiaj ntsig ntawm kev hloov pauv noob ntawm tus cwj pwm ntawm tes lossis lwm yam txheej txheem lom neeg.Qhov kev paub no tuaj yeem ua rau muaj kev nkag siab zoo ntawm cov kab mob caj ces, nrog rau txheeb xyuas cov hom phiaj kho mob.

Tsis tas li ntawd, gene-edited cell kab tuaj yeem siv los tsim thiab sim tshuaj tshiab.Los ntawm kev qhia txog kev hloov kho caj ces, cov kws tshawb fawb tuaj yeem tsim cov kab xov tooj ntawm tes uas ua rau cov kab mob sib txawv, ua rau lawv ntsuas qhov ua tau zoo ntawm kev kho mob.Qhov no tso cai rau cov txheej txheem tsim tshuaj muaj tseeb thiab ua tau zoo.

Ntxiv mus, gene-edited cell kab muaj peev xwm siv tau rau hauv cov tshuaj regenerative.Los ntawm kev hloov kho cov noob lub luag haujlwm rau kev coj tus cwj pwm ntawm tes lossis kev sib txawv, cov kws tshawb fawb tuaj yeem txhim kho cov peev txheej ntawm cov qia hlwb lossis tsim cov hlwb uas tsim nyog rau kev hloov pauv.

Qhov zoo

1. Gene-edited multi-locus nyob rau tib lub sijhawm;

2. muab kev pabcuam ib-nres los ntawm sgRNA tsim thiab synthesis rau kev kuaj kab ntawm tes;

3. Kev paub nplua nuj nyob rau hauv cov noob synthesis los pab cov noob kho cov vectors los ntawm cov txheej txheem tsim kom tiav.

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Kev Lag Luam Loj

- Gene Knock-out Cell Kab

- Gene Activation Cell Kab

- Point mutant cell kab

- Knock-hauv Cell Kab

Cov ntaub ntawv

[1]Zhang S, Shen J, Li D, Cheng Y. Cov tswv yim hauv kev xa cov Cas9ribonucleoprotein rau CRISPR/Cas9 genome kho.Theranostics.2021 Jan 1; 11(2): 614-648.doi: 10.7150/thno.47007 ib.PIB: 33391496;PIB: PMC7738854.


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